MAS-DNP applied to biomolecules and bio-materials at natural isotopic abundance Solid-state nuclear magnetic resonance (SSNMR) has been extensively used to characterize molecular structures at atomic scales. Concerning biomolecular applications, structural studies are commonly performed on 13C and/or 15N enriched samples in order to compensate for their low natural abundance (1.11 % for 13C and 0.37 % for 15N). However, this strategy is mainly restricted to biomolecules that can be easily isotopically enriched and has proven difficult to expand to other types of bio-materials. One main direction of ours is to demonstrate that MAS-DNP can provide sufficient sensitivity for experiments so that supramolecular structures can be elucidated without requiring isotopic labeling of the samples.Related contributions (see list of publications): 1- First demonstration that spatial 13C-13C proximities can be probed at natural isotopic abundance in the solid-state using DNP-enhanced NMR. See ref. 1 2- Towards Structure Determination of Self-Assembled Peptides using Dynamic Nuclear Polarization Enhanced Solid-State NMR. See ref. 7 3- Probing protein microcrystals at natural isotopic abundance using MF-DNP. See ref. 10 4- Complete 13C/15N assignment of organic molecules at natural isotopic abundance using DNP-enhanced solid-state NMR. See ref. 21
MAS-DNP applied to materials and surface studies:
Related contributions (see list of publications): 1- Application of DNP-enhanced NMR to the study of catalytic surfaces. See ref. 3, 14 2- Untangling the Self-Assembly of Organosiloxanes on Nanoparticles using 2D 29Si-29Si Solid-State NMR enhanced by Dynamic Nuclear Polarization. See ref. 13 3- Looking at bacterial cell walls with MAS-DNP enhanced NMR. See ref. 8 4- Using MAS-DNP to probe low-gamma nuclei: Structural Insights into Hydrated Y-Doped BaZrO3. See ref. 12 |
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