Matrix-Free DNP-Enhanced NMR Spectroscopy of Liposomes Using a Lipid-Anchored Biradical. Magic-angle spinning dynamic nuclear polarization
(MAS-DNP) has been proven to be a powerful technique
to enhance the sensitivity of solid-state NMR
(SSNMR) in a wide range of systems. Here, we show that
DNP can be used to polarize lipids using a lipid-anchored
polarizing agent. More specifically, we introduce a C16-functionalized biradical, which allows localization of the
polarizing agents in the lipid bilayer and DNP experiments
to be performed in the absence of excess cryo-protectant
molecules (glycerol, dimethyl sulfoxide, etc.). This constitutes
another original example of the matrix-free DNP approach
that we recently introduced. |
News & Updates >